Resources
DATASETS
Mendelsohn Lab
Transcriptomic datasets submitted to GEO
GSE123779:
WT and ShhCre;Pparg (+/-, fl/fl) whole urothelium under homeostasis
WT and ShhCre;Pparg (+/-, fl/fl) whole urothelium after UTI
WT and K5CreER; Pparg (+/-,fl/fl) sorted basal cells under homeostasis
WT and K5CreER; Pparg (+/-,fl/fl) sorted basal cells after UTI
GSE85491: RNA-SEQ of superficial, intermediate and basal layers of urothelium, post infection with UPEC
Krt5+ uninfected/Krt5+ infected
Upk+ uninfected/Upk+ infected
GSE62310: RNA-SEQ analysis of the developing lower urogenital region
E12.5 & E17.5 Urothelium
E18 Krt5+ urothelium
P5 Upk+ Urothelium
Barasch Lab
Transcriptomic datasets submitted to GEO
GSE135868: Heme metabolism confronts urinary pathogens at the urothelium.
GSE107585: Comprehensive single cell RNAseq analysis of the kidney reveals novel cell types and unexpected cell plasticity
GSE87769: Identification of Tfcp2l1 target genes in the mouse kidney
GSE87744: RNA-Seq Gene Expression Analysis in Rat Metanephric Mesenchyme overexpressing Transcription Factor Tfcp2l1
GSE87752: Genome wide map of Tfcp2l1 binding sites from mouse kidney
GSE87744: RNA-Seq Gene Expression Analysis in Rat Metanephric Mesenchyme overexpressing Transcription Factor Tfcp2l1
GSE85325: Tfcp2l1 controls cellular patterning of the collecting duct.
GSE81741: Unique Transcriptional Programs Identify Subtypes of AKI
Gharavi Lab
dbGAP Submission
phs001749.v1.p1: Microarray data from >1000 patients with CAKUT and urogenital disorders (submitted 11/27/18 related to Verbitsky et al. Nature Genetics 2019).
MOUSE RESOURCES
Mendelsohn Lab
Mendelsohn Tg(Krt5-Cre/ERT2)2lpc/J Krt5CreERT2 IGBMC
Mendelsohn B6;CBA-Tg(Upk2-icre/ERT2)1Ccc/J Upk2CreERT2 Jackson Laboratory (stock# 024768)
Mendelsohn Gt(ROSA)26Sortm4 R26RmTmG Jackson Laboratory (stock# 007576)
Mendelsohn PpargtmGonz Pparg Frank Gonzalez, NIH
Mendelsohn B6.Cg Shhtm1(EGFP/cre)Cjt/J ShhCre Jackson Laboratory (stock# 005622)
Mendelsohn Krt14CreERT2 Krt14CreERT2 Klinakis Lab
Mendelsohn Gt(ROSA)26Sortm4 (VP16/Pparg)clmJ VP16;Pparg Mendelsohn Lab
Barasch Lab
Barasch Rosa26mTmGf/+ Barasch Lab
Barasch Hmox1f/f NA Barasch
Barasch Hmox1-luc Hemoxygenase reporter mice Barasch Lab; Gift of Anupam Argawaal
Barasch Lcn2f/f; Barasch; deposited at JAX
Barasch Lcn2-Luciferase; Barasch; deposited at JAX
Barasch Slc48a1f/f; Barasch; deposited at JAX; Gift of Iqbal Hamza
Barasch Slc40a1f/f Barasch; available at JAX; Gift of Nancy Andrews
Barasch Tfr1f/f Barasch; deposited at JAX
Barasch Uprt f/f Barasch Lab; deposited at JAX; Labels RNA in vivo in specific cells at a moment in time.
Barasch Tfcp2l1f/f Barasch Lab; deposited at JAX
REAGENTS
Mendelsohn Lab
Bulk RNA-seq
UTI and WT Krt5CreERT2;mTmG 2016
UTI and WT Upk3aCreERT2;mTom 2016
E10.5 cloaca Epi Wild type embryo 2020
E10.5 cloaca Mes Wild type embryo 2020
E13.5 urothelium Wild type embryo 2020
E14.5 urothelium Wild type embryo 2019
E14.5 stroma Wild type embryo 2019
Adult Superficial cells Krt20CreERT2;mTmG 2019
SC RNA-seq
E14 Urothelium Wild type embryo 2020
E18 Whole bladder Wild type embryo 2019
Adult Urothelium Wild type embryo 2019
Adult I+S cells Upk1b Rfp+/- 2019
Adult K5-Basal Krt5CreERT2;mTmG 2019
Images:
IHC 59 Human Embryos different stages 2020
H&E 412 Scanned sections from whole embryos, 7-9 weeks 2020
Images 346 IHC and ISH 2016-
Misc:
Antibody information and protocols 20 antibodies 2019
Barasch Lab
ChuS deficient bacteria (from Dr Uhlemann)
ChuA HMA deficient bacteria (from H. Mobley)
Synthetic protocol for Pd based CO Probe that measures CO released from bladder.
Imaging protocol for Lcn2-Luc (NGAL-Luc) mice by IVIS